BCECF/AM

Code: 216254-1MG D2-231

Biochem/physiol Actions

Reversible: no

Product does not compete with ATP.

Primary TargetMonitor intracellular pH changes

Cell permeable: yes

G...


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Your Price
€213.30 1MG
€262.36 inc. VAT

Biochem/physiol Actions

Reversible: no

Product does not compete with ATP.

Primary TargetMonitor intracellular pH changes

Cell permeable: yes

General description

A variable mixture of cell-permeable ester derivatives of BCECF (Cat. No. 216252) that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules. Typical loading buffer contains 1-10 µM BCECF/AM in serum-free medium that is free of amino acids and buffers containing primary or secondary amines.

A variable mixture of cell-permeable ester derivatives of BCECF that are hydrolyzed by cytosolic esterases to yield intracellularly trapped indicator BCECF. Has been used to monitor intracellular pH changes in mammalian fibroblasts, gastric cells, lymphocytes, myocytes, and distal convoluted tubules.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Futsaetner, C.M., et al. 1993. Can. J. Microbiol. 39, 180.Hayashi, H., et al. 1992. Am. J. Physiol. 262, C628.Sasaki, S., et al. 1992. Biochim. Biophys. Acta 1137, 45.Miyata, H., et al. 1989. Biochem. Biophys. Res. Commun. 163, 500.Weiner, I.D., and Hamm, L.L. 1989. Am. J. Physiol.256, F957.

Packaging

Packaged under inert gas

1 mg in Glass bottle

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 week at -20°C.

Warning

Toxicity: Irritant (B)

assay≥85% (mixture of isomers, HPLC)
colorwhite to off-white
fluorescenceλex 500-504 nm, λem 525-531 nm
formsolid
manufacturer/tradenameCalbiochem®
Quality Level100
shipped inambient
solubilityDMF: soluble, acetonitrile: 25 mg/mL, DMSO: soluble
storage conditionprotect from light, OK to freeze
storage temp.−20°C
Cas Number117464-70-7
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