Aphidicolin

Biochem/physiol Actions

Reversible: no

Product does not compete with ATP.

Cell permeable: yes

Primary TargetDNA polymerase α, DNA polymerase δ

General description

A cell-permeable tetracyclic diterpene antibiotic. Cell synchronization agent. Blocks the cell cycle at the early S-phase. Specific inhibitor of DNA polymerase α and δ in eukaryotic cells and in some viruses of animal origin. Potentiates apoptosis induced by arabinosyl nucleosides in leukemia cell lines. Also induces apoptosis in HeLaS3 cells, but inhibits vincristine-induced apoptosis in the p53-negative human prostate cancer cell line PC-3.

A cell-permeable tetracyclic diterpene antibiotic that acts as a cell synchronization agent. Blocks the cell cycle at early S-phase. Specific inhibitor of DNA polymerase α and δ in eukaryotic cells and in some viruses. Potentiates apoptosis induced by arabinosyl nucleosides in leukemia cell lines. Also induces apoptosis in HeLa S3 cells, but inhibits vincristine-induced apoptosis in the p53-negative human prostate cancer cell line PC-3. Also available as a 30 mM solution in DMSO (Cat. No. 504744).

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Borner, M.M., et al. 1995. Cancer Res.55, 2122.Poluha, W., et al. 1995. Oncogene 10, 185.Urbani, L., et al. 1995. Exp. Cell Res. 219, 159.Schimke, R.T., et al. 1994. Philos. Trans. R. Soc. London. B. Biol. Sci.345, 311.Kuwakado, K., et al. 1993. Biochem. Pharmacol. 46, 1909.Hubermann, J.A. 1981. Cell23, 647.

Packaging

1 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.

Warning

Toxicity: Standard Handling (A)