Application

This Anti-p38/SAPK2A Antibody is validated for use in WB for the detection of p38/SAPK2A.

General description

The p38 family refers to the SAPK2/3 Kinases (Stress-Activated Protein Kinases), a subfamily of JNK/SAPK family. p38/SAPK Kinases are also activated by stresses, most notably inflammatory cytokines, irradiation, and certain toxins such as anisomycin and arsenite. SAPKs have been implicated in many pathological conditions, including inflammation, cancer and neurodegenerative diseases. As with the other MAPK family members (ERK, JNK, p38MAPK, and ERK5), p38 is activated through phosphorylation on both threonine and tyrosine residues at the Thr-X-Tyr dual phosphorylation motif, where X is Gly for p38. Once phosphorylated and active, it then goes on to phosphorylate downstream targets in both the cytoplasm and the nucleus. Some of their main targets of p38 are the kinase MAPKAPK2 and the transcription factor ATF2 that are activated via their phosphorylation.

Immunogen

KLH-conjugated linear peptide corresponding to the protein kinase domain of human p38/SAPK2A.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in human brain tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected p38/SAPK2A on 10 µg of human brain tissue lysate.

Specificity

This antibody recognizes p38/SAPK2A at the protein kinase domain.

Target description

~41 kDa observed. An uncharacterized band appears at ~35 kDa in some lysates.