Application

Immunohistochemistry Analysis: 1:200 dilution from a previous lot detected Zic2 on mouse cerebellum and hippocampus tissue.

Anti-Zic2 Antibody is an antibody against Zic2 for use in WB, IH.

General description

Zinc-finger transcription factor (Zic2) is a conserved signaling protein with critical roles in neural development including neurulation, anteroposterior patterning, neural crest formation, cerebellar development, and ipsilateral axonal targeting across the optic chiasm. It activates the transcription of target genes by DNA binding and recruitment of RNA helicase A (RHA). Zic2 has been shown to operate as a transcriptional repressor of cellular proliferation and neurogenesis. Zic2 is expressed in pluripotent cells in the blastocyst and adult brain expression overlaps with markers of neurogenesis. The Zic2kd/kd mice show holoprosencephaly and spina bifida, which derive from malformations in the anterior and posterior ends of the neural tube, respectively. Due to its apparent role in the regulation of granule cell expansion, Zic2 expression is a useful marker for mature granule neurons in the developing cerebellum.

Immunogen

Epitope: This antibody recognizes Zic2 at the N-terminus region.

This antibody is generated from rabbits immunized with a GST-tagged recombinant protein corresponding to residues in the N-terminus of mouse Zic2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in mouse cerebellum lysate.

Western Blot Analysis: : 0.1 µg/ml of this antibody detected Zic2 on 10 µg of mouse cerebellum lysate.

Specificity

This antibody recognizes Zic2.

Target description

55 kDa