Analysis Note

ControlHepG2 cell lysate.

Application

Research CategoryCell Structure

Research Sub CategoryCytoskeletal SignalingCytoskeleton

Detect Vinculin using this Anti-Vinculin Antibody validated for use in WB.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Cell spreading and movement occur though the process of binding of cell surface integrin receptors to extracellular matrix adhesion molecules. In mammalian cells, vinculin is a membrane-cytoskeletal protein in focal adhesion plaques that is involved in linkage of integrin adhesion molecules to the actin cytoskeleton. More specifically, vinculin binds to talin at its N-terminus, which, in turn, binds to beta integrins. At its C-terminus, vinculin binds to actin, phospholipids, and paxillin-forming homodimers. The binding complex of vinculin to talin and actin serves to anchor actin filaments to the membrane, (Ezzell, 1997).

Vinculin′s ability to interact with integrins at the focal adhesion appears to be critical for control of cytoskeletal mechanics, cell spreading, and lamellipodia formation. Thus, vinculin appears to play a key role in shape control based on its ability to modulate focal adhesion structure and function.

Immunogen

Epitope: Internal Domain

KLH-conjugated synthetic peptide corresponding to an internal domain of human Vinculin.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified in buffer containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl with 0.05% sodium azide.

Antigen Affinity Purified

Quality

Evaluated on a representative lot by Western blot on HepG2 cell lysate using Anti-Vinculin.

Specificity

The antibody recognizes an internal domain of human vinculin.

Predicted to cross react with monkey, pig, cow, and horse based on sequence homology.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 124 kDa