Application

Anti-VPAC2 Antibody is an antibody against VPAC2 for use in WB, IH(P).

Immunohistochemistry Analysis: 1:400 dilution from a previous lot detected VPAC2 in normal human cerebral cortex and T cells inside the mantle zone of normal human tonsil tissues.

General description

Vasoactive intestinal polypeptide receptor 2 (VPAC2), also known as PACAP type III receptor or PACAP-R3 for short, is a class B G-protein-coupled receptor. Three distinct PACAP receptors have been identified. Of the three, only PACAP-3 has been found expressed in Sartoli cells of the testis and is coupled to G and adenylyl cyclase, thus having a potential influence on the production of cAMP. In the immune system, PACAP-R3 is expressed on stimulated CD4 T cells, and binding of T cell-derived VIP to-PACAP-R3 induces a shift toward the Th2 pathway. In addition, PACAP-R3 is an essential regulator of the circadian pacemaker of the hypothalamic suprachiasmatic nuclei (SCN).

Immunogen

KLH-conjugated linear peptide corresponding to the extracellular domain of human VPAC2 (PACAP-R3).

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected VPAC2 on 10 µg of Jurkat cell lysate.

Specificity

Other homologies: mouse (84% sequence homology).

This antibody recognizes VCAP2 (PACAP-R3) at the extracellular domain.

Target description

~52 kDa observed. An uncharacterized band appears at ~39 kDa in some lysates.