Analysis Note

ControlRaji cell lysate

Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Anti-UVRAG Antibody is an antibody against UVRAG for use in WB, IC & IP.

Immunoprecipitation Analysis: 10 µg from a representative lot immunoprecipitated UVRAG from 500 µg from Raji cell lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected UVRAG in NIH/3T3 and A431 cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

UV irradiation resistance-associated gene (UVRAG) is an essential component of the Beclin1-PI(3)KC3 lipid kinase complex which functions as an important signaling checkpoint for autophagy and tumor-cell growth. More specifically, UVRAG seems to work as a multivalent trafficking effector that controls autophagosome formation and maturation, and also controls the engaging of the fusion apparatus to the endosome. UVRAG may be a promising target for cancer therapies.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human UVRAG.

Epitope: C-terminus

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in Raji cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected UVRAG in 10 µg of Raji cell lysate.

Specificity

This antibody recognizes the C-terminus of UVRAG.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~78 kDa observed