Analysis Note

ControlHeLa nuclear extract

Application

Immunocytochemistry Analysis: A 1:50 dilution from a representative lot detected UTX in HeLa, A431, and 3T3 cells.

Research CategoryEpigenetics & Nuclear Function

Detect Histone demethylase UTX using this rabbit polyclonal antibody, Anti-UTX Antibody validated for use in western blotting & ICC.

Research Sub CategoryChromatin Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Histone demethylase UTX is also known as Lysine-specific demethylase 6A, Ubiquitously-transcribed TPR protein on the X chromosome, and Ubiquitously-transcribed X chromosome tetratricopeptide repeat protein. UTX is a histone demethylase that specifically demethylates trimethylated and dimethylated but not monomethylated histone H3 ′Lys-27′. UTX is involved in regulation of posterior development, and defects can cause Kabuki syndrome type 2 (KABUK2)

Immunogen

GST-tagged recombinant protein corresponding to human UTX.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blotting in HeLa nuclear extract.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected UTX in 10 µg of HeLa nuclear extract.

Specificity

Other homologies: Canine (99% sequence homology) and Bovine (96% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~154 kDa observed. Uncharacterized band(s) may appear in some lysates.