Analysis Note

ControlE13-14 mouse brain lysate

Application

This Anti-Tbr2 antibody is validated for use in Western Blotting and Immunohistochemistry (Paraffin) for the detection of Tbr2.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Neuroscience

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected Tbr2 in mouse cerebral cortex and mouse cerebellum tissue.
Western Blot (SNAP ID) Analysis: 0.5 µg/ml from a previous lot detected Tbr2 in 10 µg of E13-14 mouse brain lysate.
Immunohistochemistry Analysis: 1:100 dilution from a previous lot detected Tbr2 in mouse and human brain.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Eomesodermin homolog (UniProt O54839; also known as T-box brain protein 2, T-brain-2, TBR-2) is encoded by the Eomes (also known as Tbr2, C77258) gene (Gene ID 13813) in murine species. Tbr2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. Trb2 has been shown to play a role in the development of the neocortex and functions in the differentiation pathway of radial glia to post-mitotic projection neuron.

Immunogen

KLH-conjugated linear peptide corresponding to mouse Tbr2.

Epitope: Transcription Activation Domain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified chicken polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in E13-14 mouse brain lysate.

Western Blot Analysis: 0.05 µg/ml of this antibody detected Tbr2 in 10 µg of E13-14 mouse brain lysate.

Specificity

Expected to react with both spliced isoforms of murine Tbr2.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~85/95 kDa observed. Target bands appear larger than the calculated molecular size of 74.8 kDa (isoform 1) and 72.7 kDa (isoform 2).