Anti-TTBK1

Analysis Note

ControlJurkat cell lysate

Application

Research Sub CategoryNeurodegenerative Diseases

Research CategoryNeuroscience

Immunohistochemistry Analysis: 2.5 µg/mL of this antibody from a representative lot detected. TTBK1 in mouse brain tissue.

This TTBK1 antibody is validated for use in WB & IHC for the detection of the TTBK1 protein.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Tau tubulin kinase (TTBK1) belongs to the casein kinase 1 superfamily and is involved in the phosphorylation of specific serine/threonine residues in paired helical filaments of the tau protein. It is specifically expressed in the brain and induces tau aggregation in human neuronal cells in a dose-dependent manner. TTBK1 levels have been found to be upregulated in the brains of Alzheimer's disease (AD) patients, and mice expressing human TTBK1 protein showed significant age-dependent memory impairment. These mice displayed increased levels of the CDK5 activators p25 and p35, and reduced levels of the NMDA receptor types 2B and 2D, suggesting that TTBK1 may play a role in memory dysfunction in AD patients. Multiple isoforms of TTBK1 are known to exist.

Immunogen

KLH-conjugated linear peptide corresponding to human TTBK1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit polyclonal in buffer containing PBS and 0.02% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blot in Jurkat cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected TTBK1 in 15 µg of Jurkat cell lysate.

Specificity

This antibody is predicted to not cross-react with TTBK2.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~143 kDa observed