Application
Anti-TRPA1 Antibody is an antibody against TRPA1 for use inWestern Blotting and Immunohistochemistry.
Immunohistochemistry Analysis: A 1:50-1,000 dilution from a representative lot detected TRPA1 in rat cerebellum, rat hindbrain, and rat pancreas tissue.
General description
Transient receptor potential A1 (also known as Transient receptor potential ankyrin 1) is an excitatory ion channel on primary sensory nerves of the pain pathway. It is encoded by the TRPA1/ANKTM1 gene in human (Trpa1/Anktm1 in rat & mouse) and plays a critical role in pain associated with tissue injury, inflammation, and nerve lesions. TRPA1 is reported to colocalize with PAR2 and coexpress with TRPV1 (Transient receptor potential vanilloid 1) in rat dorsal root ganglion (DRG) neurons. Agonist-induced TRPA1 current is shown to be enhanced upon PAR2 cleavage/activation by proinflammatory trypsin & tryptase (PMID 17571167). TRPA1 activation, in turn, is reported to result in enhanced TRPV1 phosphorylation and activity in a calcium- and PKA-dependent manner (PMID 24275229).
Immunogen
KLH-conjugated linear peptide corresponding to the cytoplasmic domain of human TRPA1.
Other Notes
Concentration: Please refer to lot specific datasheet.
Quality
Evaluated by Western Blotting in rat brain membrane tissue lysate.
Western Blotting Analysis: 0.1 µg/mL of this antibody detected TRPA1 in 10 µg of rat brain membrane tissue lysate.
Target description
~130 kDa observed. Uncharacterized band(s) may appear in some lysates.
This product has met the following criteria to qualify for the following awards: