Application

Research CategoryApoptosis & Cancer

Anti-TMIGD1 Antibody is an antibody against TMIGD1 for use in Western Blotting, Immunohistochemistry, Immunoprecipitation, Immunocytochemistry.

Research Sub CategoryAdhesion (CAMs)

Western Blotting Analysis: A 1:500 dilution from a representative lot detected TMIGD1 in 10 µg of human kidney tissue lysate and Raji membrane extract.
Western Blotting Analysis: A representative lot detected the overexpression of a c-myc tagged murine TMIDG1 in transfected HEK-293 cells, as well as the endogenous TMIDG1 in mock-transfected HEK-293 cells. Immunogen peptide blocking abolished the target band (Arafa, E., et al. (2015). Am. J. Pathol. In press).
Western Blotting Analysis: A representative lot detected a downregulated TMIGD1 level in HK-2 (human kidney 2) proximal tubular cells following TMIGD1 siRNA transfection or hydrogen peroxide treatment. Pretreatment with proteasome inhibitor Bortezomib (Cat. No. 504314) prevented H2O2-induced TMIGD1 downregulation (Arafa, E., et al. (2015). Am. J. Pathol. In press).
Western Blotting Analysis: A representative lot detected a ~45 kDa glycosylated TMIGD1 band in untreated HK-2 (human kidney 2) proximal tubular cell lysate, as well as a ~29 kDa deglycosylated TMIGD1 band in PNGase-F-treated HK-2 cell lysate (Arafa, E., et al. (2015). Am. J. Pathol. In press).
Immunohistochemistry Analysis: A representative lot detected a high TMIGD1 expression in mouse kidney proximal and distal tubular cells, while little TMIGD1 immunoreactivity was seen in podocytes (Arafa, E., et al. (2015). Am. J. Pathol. In press).
Immunoprecipitation Analysis: A representative lot immunoprecipitated highly ubiquitinated TMIGD1 from hydrogen peroxide-treated HK-2 (human kidney 2) proximal tubular cells (Arafa, E., et al. (2015). Am. J. Pathol. In press).
Immunocytochemistry Analysis: A representative lot detected the overexpression of a c-myc-tagged murine TMIDG1 in transfected HEK-293 cells by fluorescent immunocytochemistry staining (Arafa, E., et al. (2015). Am. J. Pathol. In press).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Transmembrane and immunoglobulin domain-containing protein 1 (UniProt Q6UXZ0) is encoded by the TMIGD1 (also known as TMIGD) gene (ORF UNQ9372/PRO34164; Gene ID 388364) in human. TMIGD1 protein is an adhesion molecule expressed in renal tubular epithelial cells and is highly conserved between human and murine species. TMIGD1 regulates renal epithelial cell trans-epithelial electric resistance, permeability, migration and cell morphology, and protects renal epithelial cells from oxidative stress and nutrient deprivation-induced injury. Hydrogen peroxide is shown to induce TMIGD1 ubiquitination and degradation. In addition, TMIGD1 expression is downregulated in mouse acute kidney injury (AKI) and in deoxy-corticosterone acetate (DOCA) and sodium chloride (DOCA-salt)-induced chronic hypertensive kidney disease models. TMIGD1 is produced with a signal peptide sequence (a.a. 1-29), the removal of which yields the mature single-transmembrane (a.a. 221-241) protein with a large extracellular region (a.a. 30-220) and a short cytoplasmic tail (a.a. 242-262). The extracellular region contains two C2-type Ig-like domains (a.a. 30-114 & 122-207) that mediate TMIGD1 dimerization.

Immunogen

Epitope: First C2-type Ig-like domain.

Linear peptide corresponding to a sequence from the first C2-type Ig-like domain of human TMIGD1.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit polyclonal antibody serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in H1299 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected TMIGD1 in 10 µg of H1299 cell lysate.

Specificity

This polyclonal antiserum detects glycosylated as well as unglycosylated and deglycosylated TMIGD1.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~30/39/52 kDa observed. Target bands appear larger than the calculated molecular weights of 29.19/25.82 kDa (pro-/mature form of isoform 1) and 24.28/20.91 kDa (pro-/mature form of isoform 2) due to glycosylation. Uncharacterized band(s) may appear in some lysates.