Application

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Neuroscience

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected Syntaphilin in human brain tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected Syntaphilin in human brain cells.

Anti-Syntaphilin Antibody is an antibody against Syntaphilin for use in western blotting, IHC & immunofluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Syntaphilin (SNPH) is a single-pass membrane protein that was initially identified in a yeast two-hybrid screen with the carboxy terminal region of Syntaxin-1 as bait. Syntaxin-1 is a key component of the synaptic vesicle docking machinery that forms the SNARE complex with synaptobrevin and SNAP-25. Syntaphilin competes with SNAP-25 for binding to syntaxin-1 and inhibits the formation of the SNARE complex, thereby potentially regulating synaptic vesicle exocytosis. Syntaphilin also binds dynamin-1 and inhibits dynamin-dependent endocytosis. Mice lacking syntaphilin show an increased level of mitochondrial motility and a reduced density of axonal mitochondria. This correlates with an enhanced short-term facilitation and significant impairments in motor ability, suggesting syntaphilin plays a major role in presynaptic function.

Immunogen

KLH-conjugated synthetic peptide corresponding to the center region of human Syntaphilin.

Epitope: center region

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in rat brain tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected Syntaphilin in rat brain tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~86 kDa observed. Uncharacterized bands may be observed in some lysate(s). Despite its predicted molecular weight, Syntaphilin usually migrates at higher molecular weight in SDS-PAGE.