Analysis Note

ControlHEK293 cell lysate

Application

Anti-Spred-1 Antibody is an antibody against Spred-1 for use in WB.

Research CategorySignaling

Research Sub CategoryGrowth Factors & Receptors

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Spred1 is a tyrosine kinase substrate which has a cysteine-rich domain which resembles the SPR domain at the carboxy terminus. Spred1 associated with Ras, blocks phosphorylation of Raf and disrupts MAP kinase signaling during the differentiation of neuronal cells and myocytes. A mutation in Spred1 has been linked to Lugius syndrome, an autosomal dominant RAS-MAPK pathway syndrome.

Immunogen

KLH-conjugated linear peptide corresponding to human Spred-1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HEK293 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected Spred-1 on 10 µg of HEK293 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~50 kDa observed