Analysis Note

ControlHEK293 cell lysate

Application

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Spartin in 10 µg of mouse brain tissue lysate and PC12 cell lysate.

Western Blotting Analysis: A representative lot detected Spartin in HeLa, NSC34, and PC12 cell lysates (Edwards, T. L., et al. (2009). Biochem J. 423(1):31-39.).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Spartin in HeLa cell lysate (Edwards, T. L., et al. (2009). Biochem J. 423(1):31-39.).

Immunofluorescence Analyis: A representative lot detected Spartin in HeLa cells (Edwards, T. L., et al. (2009). Biochem J. 423(1):31-39.).

Research CategoryNeuroscience

Detect Spartin using this rabbit polyclonal antibody, Anti-Spartin Antibody validated for use in western blotting.

Research Sub CategoryDevelopmental Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Spartin is also known as Spastic paraplegia 20 protein and Trans-activated by hepatitis C virus core protein 1. Spartin has been implicated in endosomal trafficking and microtubule dynamics. Spartin is ubiquitously expressed, but the highest expression found in adipose tissue. Aberrant spartin can cause spastic paraplegia autosomal recessive type 20 (SPG20).

Immunogen

Recombinant protein corresponding to human Spartin.

Physical form

Rabbit polyclonal serum containing 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in HEK293 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Spartin in 10 µg of HEK293 cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~78 kDa observed