Application

Western Blotting Analysis: A representative lot of this antibody detected S1P lyase in 30 µg of lysate from various mice tissues (Bektas, M., et al. (2010). Journal of Biological Chemistry Vol. 285, NO. 14, pp. 10880–10889)

This Anti-S1P lyase Antibody is validated for use in Western Blotting for the detection of S1P lyase.

Research Sub CategoryLipid Metabolism & Weight RegulationLipid Signaling

Research CategorySignalingMetabolism

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

S1P lyase is an important enzyme in lipid, steroid, and ceramide metabolism. S1P lyase 1 specifically cleaves phosphorylated sphingoid bases such as sphingosine-1-phosphate into fatty aldehydes and phosphoethanolamine. S1P lyase also plays roles in apoptosis with regards to regulation of ceramide production and plays roles in cell differentiation via its role in androgen and estrogen metabolism as well as that of other important lipids and bioactive lipid derivatives. S1P lyase is expressed in the endoplasmic reticulum in many cells. Interestingly, because Sphingosine-1-phosphate (S1P) is a bioactive sphingolipid that promotes cardiomyocyte survival and contributes to ischemic preconditioning, its catabolism by S1P lyase is very important in recovery from heart attacks or other ischemia related events, and thus S1P lyase is being examined as a potential new therapeutic target for reperfusion therapy in heart disease.

Immunogen

Epitope: cytoplasmic domain

Linear peptide corresponding to the cytoplasmic domain of Mouse S1P lyase.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal in buffer with 0.05% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blotting in mouse lung tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected SP-lyase1 in 10 µg of mouse lung tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 56 kDa observed