Analysis Note

ControlHuman spleen tissue lysate

Application

Research CategoryApoptosis & Cancer

Immunohistochemistry Analysis: A 1:50 dilution of this antibody detected SCARB1 in human kidney and human spleen tissues.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected SCARB1 in human spleen cells.

Optimal working dilutions must be determined by end user.

Research Sub CategoryApoptosis - Additional

Anti-SCARB1 Antibody is a highly specific rabbit polyclonal antibody, that targets Scavenger receptor class B member 1 & has been tested in western blotting, IHC & Immunofluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Scavenger receptor class B member 1 (SCARB1), also known as SR-BI, is part of the scavenger receptor superfamily, which is composed of many members with diverse structures, expression patterns, and functions. SCARB1 is a multi-ligand cell-surface receptor that mediates the selective uptake of lipid from HDL cholesterol into cells and is expressed in steroidogenic tissues in adult animals. Other ligands of SCARB1 include native, acetylated, or oxidized LDL and anionic phospholipids. SCARB1-deficient mice have elevated HDL levels and increased susceptibility to atherosclerosis on fat feeding, indicating its importance in the regulation of cholesterol homeostasis. Along with CLDN1, LDL-R, and the tetraspanin superfamily member CD81, SCARB1 has been reported to be an entry factor for the Hepatitis C virus. At least two isoforms of SCARB1 are known to exist.

Immunogen

KLH-conjugated linear peptide corresponding to human SCARB1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected SCARB1in 15 µg of human spleen tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~60 kDa observed. Uncharacterized bands may appear in some lysates. Uniprot describes 4 isoforms between ~45 kDa and ~61 kDa