Application

This SAPAP4 antibody is validated for use in WB & IHC for the detection of the SAPAP4 protein.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected SAPAP4 in rat hippocampus tissue.

General description

SAP90/PSD-95-associated protein 4 (SAPAP4, also known as DLGAP4) is a member of a protein family whose members specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. Like the other SAPAP proteins, SAPAP4 is thought to be an adaptor protein that also interacts with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP4 mRNA is targeted to cell bodies in a similar manner to SAPAP1 and -2, whereas SAPAP3 mRNA is detected mainly in cell bodies. At least three isoforms of SAPAP4 are known to exist.

Immunogen

KLH-conjugated linear peptide corresponding to human SAPAP4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Quality

Evaluated by Western Blot in SK-N-SH cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected SAPAP4 in 15 µg/mL of SK-N-SH cell lysate.

Specificity

This SAPAP4 antibody will not cross-react with other SAPAP proteins.

Target description

~138 kDa observed. The calculated molecular weight of this protein is 109 kDa, but can be observed at ~138 kDa due to post translational modification.