Analysis Note

ControlYeast extract

Application

Anti-SAP30 (yeast) Antibody is a Rabbit Polyclonal Antibody for detection of SAP30 (yeast) also known as Sin3 corepressor complex subunit SAP30 & has been validated in WB.

Research CategoryEpigenetics & Nuclear Function

Research Sub CategoryHistonesChromatin Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

SAP30 (Sin3A-associated protein) is a widely expressed nuclear protein that is functionally conserved between human and yeast. It is a component of the multiprotein histone deacetylase complex containing the transcriptional repressor Sin3, multiple histone deacetylases (HDAC1 and HDAC2), histone binding proteins (RbAp46 and RbAp48) and other polypeptides. SAP30 can recruit the deacetylase complex to promoter DNA in a Sin3 independent manner and is involved in the recruitment of the mSin3 complex to a specific subset of N-CoR corepressor complexes. The latter function suggests a role for SAP30 in N-CoR-mediated repression. Furthermore, SAP30 may be involved in imparting target specificity to the deacetylase complex.

Immunogen

Epitope: a.a. 141-157

Immunogen was a synthetic peptide corresponding to a.a. 141-157 of yeast SAP30, conjugated to KLH.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Protein A purified IgG in 0.1M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% NaN3.

Format: Purified

Quality

Western Blot Analysis:
1 µg/ml of this antibody detected SAP30 in a yeast extract.

Specificity

Recognizes yeast SAP30 at ~27 kDa.

Not tested in other species.

Storage and Stability

Stable for 1 year at 2-8°C from date of shipment.

Target description

~27 kDa