Analysis Note

ControlMouse thymus tissue lysate

Application

Research Sub CategoryImmunological Signaling

Detect S1P1 using this rabbit polyclonal antibody, Anti-S1P1 Antibody validated for use in western blotting & IHC.

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected S1P1 in mouse thymus tissue.

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Sphingosine-1-phosphate receptor 1 (S1PR1, S1P1), also known as Endothelial differentiation G-protein coupled receptor 1 (EDG1, ECGF1), Sphingosine 1-phosphate receptor Edg-1, S1P receptor Edg-1, is a bioactive lysophospholipid that evokes diverse physiological effect on most types of cells and tissues. Recent studies suggest that S1P1 promotes lymph node egression by overcoming retention signals mediated by CCR7 and G alpha i-protein-coupled receptor signaling. S1P1 may be involved in the processes that regulate the differentiation of endothelial cells. S1P1 is expressed highly in endothelial cells, and to a lesser extent in vascular smooth muscle cells, fibroblasts, melanocytes and cells of epithelioid origin.

Immunogen

KLH-conjugated linear peptide corresponding to human S1P1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in mouse thymus tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected S1P1 in 15 µg of mouse thymus tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~42 kDa observed. Uncharacterized bands may appear in some lysate(s).