Analysis Note

ControlUndifferentiated H9 human embryonic stem cells.

Application

Detect Rex-1 using this Anti-Rex-1 Antibody validated for use in western blotting & ICC.

Research CategoryStem Cell Research

Immunocytochemsitry Analysis: A 1:100 dilution of this antibody detected Rex-1 in a undifferentiated H9 human embryonic stem cells.

Research Sub CategoryPluripotent & Early Differentiation

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The Rex-1 (Zfp-42) gene, which encodes an acidic zinc finger protein, is expressed at high levels in embryonic stem (ES) and F9 teratocarcinoma cells. The Rex-1 promoter is regulated by specific octamer family members in early embryonic cells, and is involved in retinoic acid (RA)-associated reduction in expression. Rex-1 is Involved in self-renewal property of ES cells and may be involved in transcriptional regulation of pluripotency signaling pathways. Rex-1 is also involved in the reprogramming of X-chromosome inactivation during the acquisition of pluripotency and is required for efficient elongation of TSIX, a non-coding RNA antisense to XIST.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of Human Rex-1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Purified rabbit Polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blotting in a undifferentiated H9 human embryonic stem cell lysate. Western Blotting Analysis: 1.0 µg/mL of this antibody detected Rex-1 in undifferentiated H9 human embryonic stem cells.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~35 kDa observed. Uncharacterized band(s) may appear in some lysates.