Analysis Note

ControlMouse spleen tissue lysate

Application

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Anti-RanBP10 Antibody is an antibody against RanBP10 for use in Western Blotting, Immunoprecipitation.

Western Blot Analysis: A representative lot detected RanBP10 in 10 µg of HEK293 cells transfected with HA-epitope-tagged RanBP10 (wt) or Delta-44N (Delta44) mutant plasmids. (Image courtesy from the laboratory of Dr. Harald Schulze, Charite - CCM.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in HEK293 cells. (Dr. Harald Schulze, Charite - CCM.)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Ran-binding protein 10 (RanBP10) is a cytoplasmic protein that is highly expressed in megakaryocytes, which plays an essential role in platelet formation. RanBP10 functions as a guanine nucleotide exchange factor that regulates the activity of the Ran GTPase protein, and indirectly plays a role in Ran-mediated microtubule organization during the cell cycle. Ran activity facilitates proper structure and function of platelets, and mice lacking RanBP10 have abnormally shaped platelets and slightly lower levels of platelets, although this does not appear to have any significant physiological effects.

Immunogen

KLH-conjugated linear peptide corresponding to mouse RanBP10.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal serum in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in mouse spleen tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected RanBP10 in 10 µg of mouse spleen tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~68 kDa observed. An uncharacterized band appears at ~280 kDa in some lysates.