Analysis Note

ControlHuman kidney tissue lysate

Application

Research CategoryNeuroscience

Detect HIF-1 responsive protein RTP801 using this rabbit polyclonal antibody, Anti-RTP801 Antibody validated for use in western blotting & IHC.

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected RTP801 in mouse kidney tissue.

Research Sub CategoryDevelopmental Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

HIF-1 responsive protein RTP801, also known as DNA damage-inducible transcript 4 protein, is a member of the DDIT4 family regulating cell growth, proliferation and survival via inhibition of the activity of the mammalian target of rapamycin complex 1 (mTORC1). In response to energy stress, RTP801 inhibits mTOR function, resulting in dephosphorylation of downstream targets such as ribosomal protein S6 kinase 1 and 4EBP1 and decreasing cell growth. RTP801 is widely expressed with lowest levels in brain, skeletal muscle, and intestine.

Immunogen

KLH-conjugated linear peptide corresponding to human RTP801.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected RTP801 in 15 µg of human kidney tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~25 kDa observed. Uncharacterized bands may appear in some lysates.