Analysis Note

ControlHEK293 cell lysate

Application

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Protocadherin-10 in human cerebellum, thalamus, and testis tissues.

Research CategoryCell Structure

Detect Protocadherin using this rabbit polyclonal antibody, Anti-Protocadherin-10 Antibody validated for use in western blotting & IHC (Paraffin).

Research Sub CategoryECM Proteins

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Protocadherin-10 is a Single-pass type I membrane protein that is likely involved in calcium-dependent cell-adhesion. Protocadherin-10 is moderately expressed in brain testis and ovaries, with low expression in most other tissues.

Immunogen

KLH-conjugated linear peptide corresponding to the Cadherin 6 domain of human Protocadherin-10.

Epitope: Cadherin 6 domain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evlauted by Western Blotting in HEK293 cell lysates.

Western Blotting Analysis: 2 µg/mL of this antibody detected Protocadherin-10 in 10 µg of HEK293 cell lysates.

Specificity

This antibody recognizes the Cadherin 6 domain of Protocadherin-10.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~130 kDa observed. The calculated molecular weight of this protein is 113 kDa However, Uniprot states that this protein is glycosylated, which may result in higher molecular weights. Uncharacterized band(s) may be observed in some cell lysates.