Application

Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected PSAT1 in A431, HeLa, HepG2, and NIH/3T3 cells.

Research Sub CategoryEnzymes & Biochemistry

Research CategoryApoptosis & Cancer

This Anti-PSAT1 Antibody is validated for use in Western Blotting, Immunocytochemistry for the detection of PSAT1.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Phosphoserine aminotransferase (EC 2.6.1.52; UniProt Q9Y617; also known as Phosphohydroxythreonine aminotransferase, PSAT) is encoded by the PSAT1 (also known as NLS2, PSA, PSATD) gene (Gene ID 29968) in human. PSAT1 is a class-V pyridoxal-phosphate-dependent aminotransferase family member that catalyzes the reversible conversion of 3-phosphohydroxypyruvate and to 1-phosphoserine, as well as the conversion of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to 4-phosphonooxy-L-threonine, using, in both cases, L-glutamate as an amine donor, which is converted to 2-oxoglutarate. PSAT1 is involved in serine biosynthesis from glycolytic intermediates and PSAT1 upregulation is reported in non-small cell lung cancer (NSCLC) and colon cancer cells, while PSAT1 knockdoown is shown to suppress the proliferation of MDA-MB-468 and BT-20 breast cancer cells.

Immunogen

KLH-conjugated linear peptide corresponding to a sequence within the region between the two pyridoxal phosphate-binding domains of human PSAT1.

Epitope: Region between the two pyridoxal phosphate-binding domains.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in Jurkat cell lysate.

Western Blotting Analysis: 0.2 µg/mL of this antibody detected PSAT1 in 10 µg of Jurkat cell lysate.

Specificity

The immunogen sequence is 100% conserved in murine PSAT1 (UniProt Q99K85) and present in both spliced isoforms of human PSAT1 (Uniprot Q9Y617).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~40 kDa observed. 40.42 kDa (human isoform 1; Alpha) and 35.19 kDa (human isoform 2; Beta), 40.47 kDa (mouse) calculated.