Application

Research CategoryStem Cell Research

This Anti-PRDM14 Antibody is validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP) and ChIP-seq for the detection of PRDM14 .

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory on H1 human ES cells (CHAN et al., 2013. STEM CELLS, 31:682–692)

ChIPseq Analysis: A representative lot was used by an independent laboratory on H1 human ES cells (Chia et al., 2010. Nature. 316, VOL 468)

Research Sub CategoryPluripotent & Early Differentiation

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PRDM14 is an approximately 65 kDa transcriptional inhibitor that contains one histone methylating PR/SET domain (aa 266 - 367) and six zinc finger repeats (aa 400 - 568). PRDM14 is preferentially expressed in germ cells and undifferentiated embryonic stem cells in which it plays a central role in lineage specification, maintenance of pluripotency, and repression of somatic gene transcription. PRDM14 is upregulated in breast cancer and lymphoblastic leukemia. Recently, PRDM14 ensures naive pluripotency through dual regulation of signaling and epigenetic pathways in embryonic stem cells

Immunogen

Epitope: N-terminus

Human recombinant PRDM14.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Raw rabbit polyclonal serum

Format: Unpurified

Unpurified

Quality

Evaluated by Western Blotting in Human ES cell cell lysate. Western Blot Analysis: 1 µg/mL of this antibody detected PRDM14 in undifferentiated human ES cell lysate.

Specificity

Human

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~64 kDa observed. Uncharacterized bands may be observed in some lysate(s).