Analysis Note

ControlRat brain tissue lysate

Application

Research Sub CategoryNeurodegenerative Diseases

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected PHOX2A in human brain tissue.

Research CategoryNeuroscience

Anti-PHOX2A Antibody is a highly specific rabbit polyclonal antibody, that targets Phox2a & has been tested in western blotting & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Paired mesoderm homeobox protein 2A (PHOX2A), also known as ARIX1 homeodomain protein (ARIX) and Paired-like homeobox 2A (PMX2A), along with the closely related PHOX2B, is a paired-homeodomain transcription factor that maintains the noradrenergic phenotype during embryogenesis. PHOX2 proteins help regulate endogenous hydroxylases in neural crest cells and aid in sympathetic neuron generation. PHOX2A controls only the differentiation of the main noradrenergic center of the human brain, while PHOX2B is required for the differentiation of all central and nonperipheral noradrenergic centers. PHOX2A may be involved in regulating specific expression of catecholamine biosynthetic genes. PHOX2A is linked to congenital fibrosis of extraocular muscles type 2 (CFEOM2). PHOX2 regulation may have therapeutic applications for aging or degenerative noradrenergic neuron disorders.

Immunogen

KLH-conjugated linear peptide corresponding to human PHOX2A.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting/Peptide Inhibition Assay in rat brain tissue lysate.

Western Blotting/Peptide Inhibition Assay Analysis: 1µg/mL of this antibody detected PHOX2A in 15 µg of rat brain tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~32 kDa observed. Uncharacterized bands may appear in some lysate(s).