Analysis Note

ControlRaji cell lysate

Application

Immunohistochemistry Analysis: 2.5 µg/mL from a representative lot detected PDL-1 in human heart tissue.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected PDL-1 in human heart tissue.

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Anti-PDL-1 Antibody is a highly specific rabbit polyclonal antibody, that targets Programmed cell death 1 ligand 1 & has been tested in western blotting, IHC (Paraffin) & Immunofluorescence.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Programmed cell death 1 ligand 1 (PDL-1) is a B7 related protein belonging to the BTN/MOG family. PDL-1 is essential for T-cell proliferation and production of IL10 and IFNG. PDL-1 reduces the secretion of IL-2 and IL-10 from memory T cells by inhibiting cell-mediated immune responses. PDL-1 may be involved in reducing allogenic CD4+ memory T-cell responses to endothelial cells. PDL-1 is highly expressed in heart, skeletal muscle, placenta, and lung and weakly expressed in thymus, spleen, kidney, and liver.

Immunogen

KLH-conjugated linear peptide corresponding to human PDL-1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected PDL-1 in 15 µg of Raji cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~30 kDa observed. Uniprot describes 2 isoforms at ~20 kDa and 1 at ~33 kDa