Analysis Note

ControlDaudi cell lysate

Application

Immunocytochemistry Analysis: 2.5 µg/mL from a representative lot detected OTUD4 in Daudi cells.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected OTUD4 in Daudi cells.

Detect OTU domain-containing protein 4 using this rabbit polyclonal antibody, Anti-OTUD4 Antibody validated for use in western blotting, ICC & Immunofluorescence.

Research CategoryNeuroscience

Research Sub CategoryDevelopmental Neuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

OTU domain-containing protein 4 (OTUD4) is a member of the OTU (ovarian tumor) domain containing cysteine protease superfamily, in which the OTU domain generally confers deubiquitinase activity. High levels of OTUD4 are found in lung, ovary, testis, liver, heart skeletal muscle, kidney, and brain, with lower levels present in pancreas and spleen. Four isoforms of OTUD4 are known to exist, the shortest one being expressed specifically in HIV-1-infected cells.

Immunogen

KLH-conjugated linear peptide corresponding to human OTUD4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in Daudi cell lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected OTUD4 in 15 µg of Daudi cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~108 kDa observed. Uniprot describes four isoforms at ~17 kDa, ~24 kDa, ~117 kDa, and ~124 kDa Uncharacterized bands may appear in some lysates.