Application

Immunohistochemistry Analysis: A 1:50-250 dilution from a representative lot detected OSTM1 in human pancreas and human liver tissues.

Research CategoryNeuroscience

Anti-OSTM1, Cat. No. ABN1365, is a highly specific rabbit polyclonal antibody that targets Osteopetrosis-associated transmembrane protein 1 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Osteopetrosis-associated transmembrane protein 1 (UniProt: Q86WC4; also known as Chloride channel 7 beta subunit) is encoded by the OSTM1 (also known as GL) gene (Gene ID: 28962) in human. Osteopetrosis-associated transmembrane protein 1 is a single-pass type I membrane protein that is required for osteoclast and melanocyte maturation and function. It is synthesized with a signal peptide of 31 amino acids, which is cleaved to produce the mature protein. Loss of OSTM1 leads to the most severe form of osteoporosis in mice and human. Although its role in bone chemistry is well established, several studies have shown its expression in neurons, microglia, and astrocytes. OSTM1-deficiecy in the central nervous system leads to gliosis with increased astrocyte cell population and microglia activation along with retinal degeneration at 2 to 3 weeks of age in mice. (Ref.: Ref.: Heraud, C., et al. 2014. J. Biol. Chem. 289(20); 13912-25).

Immunogen

KLH-conjugated linear peptide corresponding to 18 amino acids from the C-terminus region of human Osteopetrosis-associated transmembrane protein 1 (OSTM1).

Epitope: C-terminus

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, pH 7.4 with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: 4 µg/mL of this antibody detected OSTM1 in 20 µg of human liver tissue lysate.

Specificity

This rabbit polyclonal antibody detects Osteopetrosis-associated transmembrane protein 1 in human cells. It targets an epitope within 18 amino acids from the C-terminus region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~37 kDa observed; 37.26 kDa calculated. Uncharacterized bands may be observed in some lysate(s).