Application

Research Sub CategoryNuclear Receptors

Anti-Nur77 is an antibody against Nur77 for use in WB, ICC.

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Nur77 in HEPG2 cells.

Research CategoryEpigenetics & Nuclear Function

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nurr77 is an internal nuclear receptor that is expressed developmentally and in the adult in many tissues including the liver, brain and thyroid. In the liver it acts along with other transcription factors to govern liver development as well as regeneration in the adult. Nur77 also participates in energy homeostasis by sequestrating the kinase STK11 in the nucleus, thereby attenuating cytoplasmic AMPK activation. Nur77 is also a potent pro-apoptotic protein, particularly in the brain and heart where translocation of Nur77 into the mitochondria where it induces apoptosis. Interestingly in certain cancers Nur77 overexpression is associated with pro-oncogenic activity and regulating energy levels which promotes proliferation and survival in cancer cells.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human Nur77.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in human liver tissue lysate.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected Nur77 in 10 µg of human liver tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~65 kDa observed