Anti-Neurexin-1-alpha

Analysis Note

ControlHuman cerebellum tissue lysate

Application

Detect Neurexin-1-alpha using this Anti-Neurexin-1-alpha Antibody validated for use in WB, IH(P).

Research CategoryNeuroscience

Research Sub CategoryAdhesion (CAMs)

Immunohistochemistry Analysis: A 1:100 dilution from a representative lot detected Neurexin-1-alpha in human brain tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Neurexins are heavily glycosylated and processed neuronal transmembrane cell surface proteins likely involved in cell recognition and adhesion (see review by Missler & Sudhof, 1998). Antibody Anti-Neurexin-1-alpha (ABN35) was made against a peptide immunogen corresponding to a stretch of amino acids in the extracellular domain of human Neurexin-1-alpha (Uniprot Q9ULB1). Although the theoretical full length of Neurexin-1-alpha is 161 kDa, the protein detected in human brain lysates may show a band around 160 kDa and additional bands around 45 kDa as a possible result of the posttranslational processing previously reported.

Immunogen

KLH-conjugated Linear peptide corresponding to human Neurexin-1-alpha at the extracellular laminin G-like 3 domain.

Epitope: Extracellular laminin G-like 3 domain

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human cerebellum tissue lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected Neurexin-1-alpha on 10 µg of human cerebellum tissue lysate.

Specificity

This antibody recognizes Neurexin-1-alpha at the extracellular laminin G-like 3 domain.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 45 kDa Observed At higher concentrations or longer exposures, in certain lysates, a band at ~150kD can be seen that corresponds to the expected full length Mw.
UniProtKB/Swiss-Prot Q9ULB1 (NRX1A_HUMAN) entry describes 3 isoforms produced by alternative promoter usage and alternative splicing. Reference (Accolade/Related Documents) TIG JANUARY 1998 VOL. 14 No. 1 indicates that multiple neurexin isoforms are generated from three genes by alternative promoters and alternative splicing.