Application

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot was reported to detect NuMA1 in HeLa cells.

This Anti-NuMA1 Antibody is validated for use in Western Blotting and Immunocytochemistry for the detection of NuMA1.

Research CategoryEpigenetics & Nuclear FunctionEpigenetics & Nuclear Function

Research Sub CategoryCell Cycle, DNA Replication & RepairChromatin Biology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

NuMA1 or NuMA protein is an interesting and major component of the nuclear matrix of eukaryotic cells. NuMA1 is a highly abundant protein component that appears to serve a non-structural role but is needed for mitotic spindle poles and centrosome formation as well as a linker of microtubules. These functions are important for proper cytokinesis and the setup of the proper polarity axis during cell division. NuMA1 resides in the nuclear matrix during interphase. NuMA1 dissociates from condensing chromosomes during early prophase, and relocates to the spindle poles via a dynein/dynamin complex, and it remains there until the anaphase onset. Thus NuMA1 then can be found in the nucleus during cell rest and cytoplasmically and centrosome localized during cytokinesis.

Immunogen

Three different peptides corresponding to multiple locations of NuMA1 isoform 1. Peptide, region between amino acids 1-240. Peptide 2, amino acids 1300-1500. Peptide 3, amino acids 1860-2115. Note that these peptides are also found in isoform 2.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

Antigen Affinity Purified

Quality

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1 µg/mL dilution of this antibody detected NuMA1 in a A431 cell lysate.

Specificity

Recognizes NuMA1

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~ 238 kDa observed