Application

Research CategoryEpigenetics & Nuclear Function

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected NSD2 in human cerebellum and thalamus tissues.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected NSD2 in human kidney tissues.

Immunoprecipitation Analysis: 5 µg of this antibody immunoprecipitated NSD2 from 500 µg of HCT-116 cell lysate.

Research Sub CategoryChromatin Biology

Detect NSD using this rabbit polyclonal antibody, Anti-NSD2 Antibody validated for use in western blotting, IHC & IP.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Nuclear SET domain-containing protein 2 (NSD2) is also called Histone-lysine N-methyltransferase NSD2, Multiple myeloma SET domain-containing protein (MMSET), Protein trithorax-5 and Wolf-Hirschhorn syndrome candidate 1 protein (WHSC1). NSD2 is a histone methyltransferase that acts on histone H3 ′Lys-27′ (H3K27me). Isoform RE-IIBP is thought to be a transcription regulator that uses HADC recruitment to binding DNA and suppress IL5 transcription. Chromosomal aberrations to WHSC1 are linked to multiple myeloma tumors.

Immunogen

KLH-conjugated linear peptide corresponding to a region near the C-terminus of human NSD2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected NSD2 in 10 µg of HeLa cell lysate.

Specificity

This antibody is expected to cross-react with isoforms 1 (152 kDa), 3 (71 kDa), and 4 (66 kDa, REII-BP; IL-5 promoter REII-region-binding protein) based upon immunogen design.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~150 kDa observed. Uniprot describes 7 different isoforms ranging from 30 kDa to 152 kDa