Application

This Anti-Menin/MEN1 Antibody is validated for use in Western Blotting for the detection of Menin/MEN1 .

Western Blotting Analysis: A representative lot detected endogenous Menin in TGP61 nuclear extract, as well as exogenously expressed Menin in transfected NIH3T3 cells (Jin, S., et al. (2003). Cancer Res. 63(14):4204-4210).

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Menin (UniProt O00255) is a nuclear protein encoded by the MEN1 gene (also known as MEAI, SCG2) (Gene ID 4221) in human. MEN1 is a tumor suppressor gene frequently mutated in the familial multiple endocrine neoplasia type I (MEN-1) cancer syndrome characterized by tumors in multiple endocrine organs including the parathyroid, pancreatic islets, and the pituitary. Menin mediates cell proliferation and differentiation actions of transforming growth factor-beta (TGF-beta) ligand family members. Menin is reported to associate with histone methyltransferases (HMT) MLL1 (KMT2A) and MLL4 (KMT2B) to form menin-HMT complexes in both human and murine model systems. In addition, menin is also shown to be involved in DNA repair with FANCD2.

Immunogen

KLH-conjugated linear peptide corresponding to the C-terminus of human Menin/MEN1.

Epitope: C-terminus

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Quality

Evaluated by Western Blotting in Tamoxifen treated MEF 545T and MEF 545D cell lysate.

Western Blotting Analysis: A 1:20,000 dilution of this antibody detected Menin/MEN1 in 10 µg of Tamoxifen treated MEF 545T and MEF 545D cell lysate.

Specificity

Expected to react with all three spliced variants: Isoform 1 (Long), Isoform 2 (Short), and Isoform 3.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~70 kDa observed