Application

Anti-Membralin Antibody, Cat. No. ABN1661, is a highly specific rabbit polyclonal antibody that targets Membralin and has been tested in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, and Western Blotting.

Immunoprecipitation Analysis: 1.0 µg/mL from a representative lot detected Membralin in lysate from HEK293 cells transfected with mouse membralin. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhang′s Lab at Sanford Burnham Prebys Medical Discovery Institute).

Immunocytochemistry Analysis: 10 µg/mL from a representative lot detected Membralin in mouse neural progenitor cells. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhang′s Lab at Sanford Burnham Prebys Medical Discovery Institute).

Immunohistochemistry Analysis: 5.0 µg/mL from a representative lot detected Membralin in mouse spinal cord tissues. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhang′s Lab at Sanford Burnham Prebys Medical Discovery Institute).

Western Blotting Analysis: 1.0 µg/mL from a representative lot detected Membralin in lysate from HEK293 cells transfected with mouse membralin. (Courtesy of Dr. Lu-Lin Jiang and Dr. Bing Zhu in Dr. Dongxian Zhang′s Lab at Sanford Burnham Prebys Medical Discovery Institute).

Research CategoryNeuroscience

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Membralin (UniProt: Q8CIV2; also known as Transmembrane protein 259) is encoded by the Tmem259 (also known as ORF61) gene (Gene ID: 216157) in murine species. Membralin is a highly conserved protein with several transmembrane regions. However, the sequence of membralin does not show any closely related genes. It has multiple glycosylation sites and cytosolic regions at both the N-terminal ends and C-terminal ends. It is expressed in multiple tissues, including brain and spinal cord. However, it is not found in heart, skeletal muscle, and spleen. In humans its membralin expression is reported in certain cancers, including the ovarian cancers. Membralin is reported to have a role in the ER-associated protein degradation (ERAD) pathway and is required for clearance of misfolded proteins in the endoplasmic reticulum (ER). It is also required for survival of motor neurons. Membralin mutant mice display increased basal ER stress and severe and early-onset motor neuron disease and die by postnatal day 5 or 6. (Ref.: Chen YC et al. (2005). Biochimica Biophysica Acta 1730(2): 96-102.)

Immunogen

A Myc-conjugated linear peptide from the N-terminal region of mouse membralin.

Epitope: N-terminus

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein A + Protein G

Format: Purified

Quality

Evaluated by Western Blotting in HEK293 cells overexpressing membralin.

Western Blotting Analysis: 1 µg/mL of this antibody detected Membralin in 4 µL of lysate from HEK293 cells transfected with mouse-myc-membralin and overexpressing Membralin.

Specificity

This polyclonal antibody specifically detects membralin in HEK293 cells overexpressing membralin.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~68 kDa observed; 63.56 kDa calculated. Uncharacterized bands may be observed in some lysate(s).