Application

Immunohistochemistry Analysis: A 1:500-2,000 dilution from a representative lot detected MCPH1 in human cerebellum, human testis, and human cerebral cortex tissue.

Research CategoryNeuroscience

Anti-MCPH1 Antibody is a highly specific rabbit polyclonal antibody, that targets Microcephalin & has been tested in western blotting & IHC.

Research Sub CategoryDevelopmental Signaling

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The protein named Microcephalin and encoded by the human gene named MCPH1 is a DNA/chromatin binding protein implicated in chromosome condensation and DNA damage induced cellular responses. MCPH1 plays a role in neurogenesis and is involved in the regulation of the size of the cerebral cortex. MCPH1 interacts with several proteins including H2AFX under DNA damage conditions and CDC27 during normal cell growth. MCPH1 is expressed cytoplasmically in a variety of cell types and tissues and is particularly expressed in the fetal brain, liver and kidney. Defects in MCPH1 can give rise to microcephaly a disease where the head circumference is markedly reduced and effected patients are mentally retarded, showing a high number of prophase-like cells in cytological examinations. In addition, cell divisions are irregular and demonstrate a delay in post-mitotic chromosome decondensation. Interestingly, recent research demonstrates that MCPH1 also functions as a tumor suppressor that is regulated by the microRNA miR-27a.

Immunogen

KLH-conjugated linear peptide corresponding to human MCPH1.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in human testis tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected MCPH1 in 10 µg of human testis tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~111 kDa observed. Uncharacterized band(s) may appear in some lysates.