Application

Research Sub CategoryRNA Metabolism & Binding Proteins

Anti-PCBP4 Antibody is a highly specific rabbit polyclonal antibody, that targets Poly (rC) binding protein 4 & has been tested in western blotting, ICC & Immunofluorescence.

Research CategoryEpigenetics & Nuclear Function

Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected PCBP4 in HeLa cells.

Immunofluorescence Analysis: 20 µg/mL from a representative lot detected PCBP4 in HeLa cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

PCBP4 (also known as MCG10, Poly (rC) binding protein 4 and alpha-CP4) is a member of the KH-domain RNA-binding protein family. Proteins of this family also referred to as alpha-CPs, bind to RNA with specificity for C-rich pyrimidine regions. Alpha-CPs play important roles in post-transcriptional activities and have different cellular distributions. PCBP4 is induced by p53 and DNA damage via two p53-responsive promoter elements. Its overexpression in transfected cells results in the suppression of cell proliferation by inducing apoptosis and cell cycle arrest in G2-M. Decreased expression of one isoform of PCBP4 has been observed in many forms of lung cancer, leading to speculation that PCBP4 can act as a tumor suppressor.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to a 17 amino acid peptide near the C-terminus of human PCBP4.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in HeLa cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected PCBP4 in HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~51 kDa observed. Uncharacterized bands may be observed in some lysate(s).