Application

Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected MBD5 in human pancreas and human cervical cancer tissue sections

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected MBD5 in HeLa, A431, and HEK293 cells.

Western Blotting Analysis: 2 µg/mL from a representative lot detected MBD5 in human liver tissue lysate.

Research CategoryEpigenetics & Nuclear Function

Anti-MBD5, Cat. No. ABE1322, is a highly specific rabbit polyclonal antibody that targets Methyl-CpG-binding domain protein 5 and has been tested for use in Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Methyl-CpG-binding domain protein 5 (UniProt: Q9P267; also known as Methyl-CpG-binding protein MBD5) is encoded by the MBD5 (also known as KIAA1461) gene (Gene ID: 55777) in human. MBD proteins play a role in diverse processes, including chromatin remodeling, DNA damage repair, histone methylation, and X chromosome inactivation. MBD5 is detected in heart, placenta, liver, skeletal muscle, kidney, and pancreas. It binds to heterochromatin, but does not interact with either methylated or unmethylated DNA (in vitro). It contains a MBD domain (aa 11-81) and a PWWP (Pro-Trp-Trp-Pro) domain (aa 1385-1447). Both MBD and PWWP domains are shown to be essential for chromocentric localization. Two isoforms of MBD5 have been reported that are produced by alternative splicing. However, only the isoform 1 associates with pericentric heterochromatin. Mutations in MBD5 gene are known to cause autosomal dominant mental retardation, which is characterized by significantly below average general intellectual functioning associated with impairments in adaptive behavior. (Ref.: Camarena, V et al., (2014). EMBO Mol. Med. 6(8); 1003 1015).

Immunogen

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal region of human Methyl-CpG-binding domain protein 5 (MBD5).

Epitope: unknown

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Immunohistochemistry in human prostate tissue sections.

Immunohistochemistry Analysis: A 1:250 dilution of this antibody detected MBD5 in human prostate tissue sections.

Specificity

This rabbit polyclonal antibody detects isoform 1 of human Methyl-CpG-binding domain protein 5. It targets an epitope within 16 amino acids fromt the C-terminal region.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~160 kDa observed; 159.89 kDa calculated. Uncharacterized bands may be observed in some lysate(s).