Application

This Anti-MATE2 Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of SLC47A2.

Research CategoryApoptosis & Cancer

Research Sub CategoryIon Channels & Transporters

Immunohistochemistry Analysis: A 1:250-1,000 dilution from a representative lot detected MATE2/SLC47A2 in human kidney, pancreas, and prostate tissue sections.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Multidrug and toxin extrusion protein 2 (UniProt Q86VL8; also known as hMATE-2, Kidney-specific H(+)/organic cation antiporter, MATE-2, Solute carrier family 47 member 2) is encoded by the SLC47A2 (also known as MATE2) gene (Gene ID 146802) in human. Multidrug and toxin extrusion (MATE) proteins are transporters mediating the efflux of organic cations from cells. Driven by oppositely directed proton gradient, MATEs are considered secondary active transporters. MATEs are located at the apical membrane of proximal tubular cells and work in concert with the basal organic cation transporters (OCTs) in the elimination of cationic drugs from the kidney. Human SLC47A2 gene produces two functional isoforms, the 602-a.a. canonical MATE2 (NP_690872.2; UniProt Q86VL8-1) and the shorter spliced isoform 566-a.a. MATE2-K (NP_001093116.1; UniProt Q86VL8-3) lacking a.a. 178-213 of MATE2 due to a partial deletion of exon 7-coding sequence. MATE2 and MATE2-K are preferentially localized in the kidney, while MATE2 is also detected in the placenta. MATE2 contains 13 transmembrane (a.a. 34-54, 67-87, 120-140, 154-174, 220-240, 249-269, 290-309, 328-348, 369-389, 403-423, 443-463, 467-487, 488-578) helices (TMHs), having its N-terminal end (a.a. 1-33) at the cytoplasmic side and its C-terminus (a.a. 600-602) at the extracellular side. The first twelve TMHs are required for proper transport function while the thirteenth TMH is likely needed for protein turnover.

Immunogen

Epitope: Third cytoplasmic domain.

KLH-conjugated linear peptide corresponding to a sequence from the third cytoplasmic domain of human MATE2/SLC47A2.

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified.

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in human kidney tissue lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected MATE2/SLC47A2 in 10 µg of human kidney tissue lysate.

Specificity

The immunogen sequence is present only in the canonical form (UniProt Q86VL8-1) of human SLC47A2, but not any of the other five spliced isoforms reported by Uniprot (Q86VL8-2 through Q86VL8-6).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~53 kDa observed. 65.09 kDa calculated. Smaller than predicted band size has also been reported for the MATE2-K spliced isoform and the related MATE1/SLC47A1 (Tanihara, Y., et al. (2007). Biochem. Pharmacol. 74(2):359-371). Uncharacterized band(s) may appear in some lysates.