Anti-Kinectin

Analysis Note

ControlHuman testis tissue lysate

Application

Research Sub CategoryCytoskeleton

Research CategoryCell Structure

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected Kinectin in HeLa and A431 cells.

Detect Kinectin using this Anti-Kinectin Antibody validated for use in Western Blotting, ICC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Organelle transport is facilitated by molecular motors, such as kinesin and dynein, which interact with microtubules to transport cargo in an ATP-dependent manner. The shuttling of organelles is also dependent on kinectin, a transmembrane ER protein that associates with kinesin and stimulates its ATPase activity. Kinesin-kinectin dynamics have also been linked to focal adhesion growth. In addition, kinectin has been shown to activate the RhoG GTPase pathway which involves Rac1 and Cdc42 signaling. Kinectin is expressed in testis, ovary, spleen, thymus, prostate, colon, and small intestine.

Immunogen

KLH-conjugated linear peptide corresponding to the lumenal region of human Kinectin.

Epitope: Lumenal region

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in human testis tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected Kinectin in 10 µg of human testis tissue lysate.

Specificity

This antibody recognizes the lumenal region of Kinectin.

Other homologies: Mouse (82% Mouse sequence homology) and Rat (79% sequence homology).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~158 kDa observed. Uniprot describes 3 isoforms at ~149, ~150, and ~156 kDa