Analysis Note

ControlRat heart tissue lysate

Application

Research CategoryApoptosis & Cancer

Research Sub CategoryApoptosis - Additional

Detect Importin using this rabbit polyclonal antibody, Anti-KPNA2 Antibody validated for use in western blotting & IHC.

Immunohistochemistry Analysis: 5 µg/mL from a representative lot detected KPNA2 in human heart tissue.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Karyopherin alpha nucleoprotein interactor 2 (KPNA2), also known as Importin alpha-2, RAG cohort protein 1 (RCH1), SRP1-alpha QIP2 (SRP1), is the alpha subunit 2 of karyopherin which functions as a cargo carrier that transports various complexes from cytoplasm into nucleus and forms a complex with importin subunit beta-1 and. KPNA2 is expressed ubiquitously and contains one IBB/importin beta domain, ten Armadillo repeats and three intervening nuclear localization sequences (NLSs). Recent studies have shown that KPNA2 plays an important role in tumorigenesis and tumor progression. KPNA2 is a heterodimer with KPNB1. It has been implied to interact with HIV-1 Vpr, PLAG1, ANP32E, ARL4A, CTNNBL1, NBN and APEX1 (via N-terminus). KPNA2 is also a component of a complex containing CSE1L, RAN and KPNA2 and interacts directly with CSE1L.

Immunogen

KLH-conjugated linear peptide corresponding to human KPNA2.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in rat heart tissue lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected KPNA2 in 15 µg of rat heart tissue lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~55 kDa observed. Uncharacterized bands may appear in some lysate(s).