Application

Activity Assay Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in this functionally blocking antibody abrogated AGS cell attachment to fibronectin while causing only a slight inhibition of cell attachment to vitronectin (Hutton, M., et. al. (2010). Infection and Immunity. 78(11):4523-4531).
Immunohistochemistry Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in floating villi tissue (Damsky, C. H., et al. (1992). Journal of Clinical Investigation. 89:210-222).
Immunohistochemistry Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in CTB aggregates that have invaded matrigel in vitro (Damsky, C. H., et al. (1994). Development. 120:3657-3666).
Immunocytochemistry Analysis: A representative lot detected Integrin Beta1, clone AIIB2 (Azide Free) in AGS cells (Hutton, M., et. al. (2010). Infection and Immunity. 78(11):4523-4531).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Integrin Beta1, clone AIIB2 (Azide Free) in NP-40 cell lysate (Werb, Z., et al. (1989). JCB. 109:877-889).

This Anti-Integrin Beta1, clone AIIB2 (Azide Free) Antibody is validated for use in Western Blotting and Immunocytochemistry and Activity Assay and Immunoprecipitation and Immunohistochemistry for the detection of Integrin Beta1.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Integrin beta-1, also known as Fibronectin receptor subunit beta (FNRB), and Glycoprotein IIa (GPIIA), is a transmembrane glycoprotein that forms noncovalent complexes with various alpha integrin subunits to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in cell adhesion and recognition in a variety of processes including embryogenesis, hemostasis, tissue repair, immune response and metatastatic diffusion of tumor cells. Interactions between integrins and extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression. Phosphorylation of threonines 788 and 789 on integrin beta1 receptor may play a key role in cell-cycle dependent regulation.

Immunogen

Whole cells corresponding to human Integrin Beta1, clone AIIB2 (Azide Free).

Physical form

Protein G Purified

Format: Purified

Purified rat monoclonal IgG1κ in buffer containing PBS without preservatives.

Quality

Evaluated by Western Blotting in HUVEC cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Integrin Beta1, clone AIIB2 (Azide Free) in 10 µg of HUVEC cell lysate.

Storage and Stability

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Target description

~110 kDa observed. The calculated molecular weight of this protein is 88 kDa, but can be observed at ~110 kDa due to high glycosylation.