Analysis Note

ControlA20 cell lysate

Application

Research Sub CategoryNeurodegenerative Diseases

Research CategoryNeuroscience

Detect Inositol-requiring protein 1 using this rabbit polyclonal antibody, Anti-IRE1p Antibody validated for use in western blotting, ICC & IHC.

Immunohistchemistry Analysis: A 1:1,000 dilution from a representative lot detected IRE1p in human pancreas and kidney tissues.

Immunocytochemistry Analysis: 1 µg/mL from a representative lot detected IRE1p in A20 cells.

Immunofluorescence Analysis: 2 µg/mL from a representative lot detected IRE1p in A20 cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Inositol-requiring protein 1 (IRE1p), also known as Ire1-alpha (IRE1a), Endoplasmic reticulum-to-nucleus signaling 1 (ERN1), and Serine/threonine-protein kinase/endoribonuclease IRE1, contains 1 KEN domain and 1 protein kinase domain.
IRE1p has an endoribonuclease activity that is required to splice X-box binding protein (XBP1) mRNA converting it to a potent UPR transcriptional activation. Depletion of IRE1p through the expression of a dominant negative form of IRE1p has no effect on transfected cells, but cell death via apoptosis occurs under stress conditions that cause unfolded proteins to accumulate in the ER. IRE1p is ubiquitously expressed with high levels observed in pancreatic tissue.

Immunogen

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human IRE1p.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in A20 cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected IRE1p in A20 cell lysate.

Specificity

This antibody recognizes the C-terminus of IRE1p.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~114 kDa observed. Uncharacterized bands may appear in some lysate(s).