Application

Research Sub CategoryImmunoglobulins & Immunology

Anti-IL-34 Antibody detects level of IL-34 & has been published & validated for use in IL-34.

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Interleukin-34 (IL-34) was identified through a functional screening of a library of secreted proteins after transfection of an embryonic kidney cell line with recombinant cDNAs. Flow cytometry and monocyte viability assays demonstrated increased survival and/or proliferation of monocytes induced by IL-34 and specific binding of IL-34 to CD14+ monocytes. IL-34 is a second ligand to the colony stimulating factor (CSF)-1 receptor in addition to CSF1. Like CSF1, IL-34 stimulates phosphorylation of ERK1 and ERK2 in human monocytes and induces differentiation of bone marrow cells into macrophages.

Immunogen

Epitope: N-terminus

KLH-conjugated synthetic peptide corresponding to the N-terminus of human IL-34.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Antigen Affinity Purified

Purified rabbit polyclonal in buffer containing PBS with up to 0.1% sodium azide.

Quality

Evaluated by Western Blotting in human brain tissue lysate with and without blocking peptide.

Western Blotting Analysis: 0.25 µg/mL of this antibody detected IL-34 in human brain tissue lysate with and without blocking peptide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~47 kDa observed. Despite its predicted molecular weight of 27 kDa, IL-34 will often run at higher molecular weight in SDS-PAGE.