Analysis Note

ControlHeLa cell lysate

Application

Research Sub CategoryTranscription Factors

Research CategoryStem Cell Research

Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected IKAROS in ReNcell™ human neural progenitor cells.

Anti-IKAROS Antibody detects level of IKAROS & has been published & validated for use in WB & IC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

IKAROS, also known as IKZF1, is a transcription factor that is important for the regulation of lymphopoiesis and also the development of the immune system. It has also been linked to adult and fetal erythroid differentiation in humans. IKAROS has been shown to induce local chromatin accessibility during CD4+ T cell differentiation and therefore, activating Th2 expression.

Immunogen

KLH-conjugated linear peptide corresponding to human IKAROS.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in HeLa cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected IKAROS on 10 µg of HeLa cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~65 kDa observed. (Martin-Ibanez, R., et al. (2010). The Journal of Comparative Neurology. 518:329–351.) An uncharacterized band appears at ~57 kDa in some lysates.