Application

Research CategoryApoptosis & Cancer

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected HPGD in human ileum tissue.

Research Sub CategoryApoptosis - Additional

Detect Prostaglandin dehydrogenase 1 using this rabbit polyclonal antibody, Anti-HPGD Antibody validated for use in western blotting & IHC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

The protein named HPGD, or also known as 15-PGDH, or Prostaglandin dehydrogenase 1, and encoded by the gene HPGD/PGDH1, is a protein necessary for the regulation of prostaglandin levels. Prostaglandins are potent inflammatory agents, thus their regulation is key for control inflammatory responses. HPGD works to mediate prostaglandin inactivation by catalyzing the NAD dependent dehydrogenation of lipoxin A4 to form 15-oxo-lipoin A4. Additionally, HPGD inhibits in vivo proliferation of colon cancer cells. HPGD is localized to the cytoplasm. HPGD is expressed in intestinal and colon tissues. HPGD is down regulated in colon cancer and thus is being examined as a therapeutic agent. Defects in HPGD may be associated with Hypertrophic osteoarthropathy, a disease characterized by digital clubbing, periostosis, acroosteolysis, painful joint enlargement, and variable features of pachydermia that include thickened facial skin and a thickened scalp. Other developmental anomalies include delayed closure of the cranial sutures and congenital heart disease. Other diseases associated with this gene's defects are Crainoosteoarthropathy (swollen joint disease) and congenital nail clubbing where one has very large nails on the fingers and toes.

Immunogen

KLH-conjugated linear peptide corresponding to human HPGD.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit Polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in Caco-2 cell lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected HPGD in 10 µg of Caco-2 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~32 kDa observed