Application

Flow Cytometry Analysis: 1 µg from a representative lot detected HLA-DRB3 in one million Raji cells.

This rabbit polyclonal Anti-HLA-DRB3 Antibody, Cat. No. ABD113, is a rabbit polyclonal antibody tht detects HLA class II histocompatibility antigen, DR beta 3 chain (HLA-DRB3) and has been tested for use in Flow Cytometry and Western Blotting.

Research CategoryInflammation & Immunology

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

HLA class II histocompatibility antigen, DR beta 3 chain (UniProt: P79483; also known as MHC class II antigen DRB3) is encoded by the HLA-DRB3 gene (Gene ID: 3125) in human. DRB3 is a single-pass type I membrane protein; that is synthesized with a signal peptide of 29 amino acids that is subsequently cleaved to generate a mature form. It also contains an Ig0like C1 type domain (aa 126-214). It is a heterodimer of an alpha and a beta subunit; also referred as MHC class II molecule. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the endoplasmic reticulum (ER) to form a heterononamer. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation. DRB3 binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells.

Immunogen

Epitope: N-terminus

KLH-conjugated linear peptide corresponding to 15 amino acids from the N-terminal half of human HLA class II histocompatibility antigen, DR beta 3 chain (HLA-DRB3).

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Affinity Purified

Quality

Evaluated by Western Blotting in Raji cell lysate.

Western Blotting Analysis: 2 µg/mL of this antibody detected HLA-DRB3 in 10 µg of Raji cell lysate.

Specificity

This rabbit polyclonal antibody detects HLA class II histocompatibility antigen, DR beta 3 chain in human cells. It targets an epitope within 15 amino acids from the N-terminal half.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~32 kDa observed; 29.96 kDa calculated. Uncharacterized bands may be observed in some lysate(s).