Analysis Note

ControlJurkat cell lysate

Application

Western Blot Analysis: 0.1 µg/mL from a representative lot detected HAX-1 in 10 µg of HeLa cell lysate and rat liver tissue lysate.

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected HAX-1 in NIH/3T3 and HeLa cells.

Research Sub CategoryApoptosis - AdditionalCytoskeleton

Research CategoryApoptosis & Cancer

Anti-HAX-1 Antibody is an antibody against HAX-1 for use in WB & IC.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

HAX-1 (HS1-associated protein X-1) is a mitochondrial anti-apoptotic protein and is thought to be regulated by Omi/HtrA2. HAX-1 has been found to associate with BSEP, MDR1 and MDR2 ABC-transporters and interacts with cortactin to play a role in BSEP internalization from the apical membrane. HAX-1 and alphavbeta6 are found to be highly expressed in certain oral cancers and work together to enhance tumor invasion and progression. High expression of HAX-1 in many other cancer tissues shows that HAX-1 is important in neoplastic transformation.

Immunogen

Epitope: HCLS1-, CASP9-, and GNA13-binding domains

KLH-conjugated linear peptide corresponding to the domain of human HAX-1 that is involved in HCLS1-, CASP9-, and GNA13-bindings.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blot in Jurkat cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected HAX-1 in 10 µg of Jurkat cell lysate.

Specificity

This antibody recognizes HAX-1 at the domains involved in HCLS1-, CASP9-, and GNA13-binding.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~35 kDa observed.
HAX-1 has been shown to migrate as a ~34-36 kDa band in WB (Ortiz, D.F., et al. (2004). JBC. 279(31):32761-32770).