Application

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Galectin-4 in 10 µg of Colo320 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Galectin-4 in 10 µg of human small intestine tissue lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected Galectin-4 in human stomach tissue.

This Anti-Galectin-4 Antibody is validated for use in Western Blotting and Immunohistochemistry for the detection of Galectin-4.

Research CategoryCell Structure

Research Sub CategoryAdhesion (CAMs)

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Galectin 4, or GAL-4, is another member of the Galectin family. Galectins are carbohydrate binding proteins that appear important in cell-cell contact, cell migration, and cell to cell signaling in both normal and disease (cancerous) tissues. Galectin 4 differs from its sister family members in the range of carbohydrate substrates that it binds. Galentin-4 is synthesized as a cytosolic protein, but can be externalized. During development and in adult normal tissues, galectin-4 is expressed only in the alimentary tract, from the tongue to the large intestine. It is often found in relatively insoluble complexes as a component of either adherent junctions or lipid rafts in the microvillus membrane, and it has been proposed to stabilize these structures. Strong expression of galectin-4 can be induced, however, in cancers from other tissues including breast and liver. Also, Galectin-4 appears to specifically bind CD3 on activated T-cells in the gut and regulate their activity via calpain mediated apoptosis.

Immunogen

KLH-conjugated linear peptide corresponding to human Galectin-4 near the N-terminal.

Epitope: near N-terminal

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Quality

Evaluated by Western Blotting in HT-29 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Galectin-4 in 10 µg of HT-29 cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~36 kDa observed. Uncharacterized band(s) may appear in some lysates.